There was a great deal of scientific and political discussion about these prescriptions, since there were no studies to demonstrate the effectiveness of this kit against COVID-19 [6,7,8]. Vitamin D (Vit D) is a fat-soluble vitamin and/or hormone that plays a key role in bone health and calcium homeostasis. antibodies (NAbs) in a group of elderly residents in a nursing home in northeastern Brazil. Serum levels of COVID-19 NAbs were assessed, along with vitamin D and zinc, in two phases. The first (T1) was in August 2022 with 26 elderly people, and the second (T2) was in March 2023 with 21, due to the death of five participants. Overall, we observed satisfactory levels for vitamin D, with no participants showing a deficiency in either test, and zinc, with only two participants having a negative result at T1 and three at T2. However, a drop in the average number of NAbs was observed, especially in women (T1 = 89 19 vs. T2 = 57 44), highlighting the importance of monitoring this immunological parameter in the population studied. Based on the results, we suggest that there is no synergism between the micronutrients studied and NAbs (p> 0.05). Further studies are needed to BMP2B consolidate the findings of an absence of synergism between vitamin D and zinc in the maintenance of NAbs. Keywords:antibodies, COVID-19, immunization, neutralizing, vitamin D, SARS-CoV-2, zinc == 1. Introduction == The COVID-19 pandemic, declared by the World Health Business (WHO) in 2020, has had a significant impact on the elderly populace. Worldwide, the pandemic has resulted in more than 750 million confirmed cases and almost 7 million deaths [1]. In Brazil, COVID-19 has had a major impact, with nearly 7 million cases and more than 700,000 deaths [2]. More than 80% of deaths worldwide were associated with the elderly (over 60 years of age) [3], and in Brazil, it is estimated that the elderly populace accounted for 76.6% of deaths [1]. To minimize the impact on this populace, the elderly group was classified as a priority group in the vaccination queue in Brazil and was able to receive vaccine doses in January 2021 [4]. The elderly has been considered a risk group and are more likely to develop serious complications and death than the younger populace [4]. In the search for ways to protect this populace against SARS-CoV-2 while vaccines have been developed, research has been initiated around the influence of vitamins and minerals around the coronavirus through the effect they can have around the immune system through supplementation [5]. Due to the lack of information about effective treatments for COVID-19, and in search of a way to minimize its SAR7334 impact on public health, the COVID-19 Kit was created in Brazil. This kit consisted of anti-parasitic drugs, antibiotics, anti-inflammatories, vitamins such as vitamin C and D, and minerals such as zinc. There was a great deal of scientific and political discussion about these prescriptions, since there were no studies SAR7334 to demonstrate the effectiveness of this kit against COVID-19 [6,7,8]. Vitamin D (Vit D) is usually a fat-soluble vitamin and/or hormone that plays a key role in bone health and calcium homeostasis. This vitamin is effective in stimulating the innate and adaptive immune systems [9,10]. This may be due to Vit Ds positive regulation of the antimicrobial peptide cathelicidin (LL-37). This peptide is usually involved in inhibiting bacteria by disrupting the cell membrane and affects certain viruses and fungi. LL-37 is also responsible for promoting chemotactic, immunomodulatory effects and affects serum levels of pro-inflammatory cytokines, as well as increasing autophagy in macrophages and promoting innate SAR7334 immunity against viral infections [11]. In addition, Vit D plays a role in suppressing the production of pro-inflammatory cytokines, including modulating the production of T and B lymphocytes [9,12]. Lymphocytes are also directly involved in the fight against contamination by recognizing antigens and activating macrophages, as well as in immunological memory by producing antibodies that neutralize viral access SAR7334 to host cells [13]. These antibodies are known as neutralizing antibodies (NAbs), which are also produced by B lymphocytes and are associated with the primary blocking of various viral proteins involved in the viral infection mechanism [13]. In addition to vitamins, the metal.