After Cell growthinRPMI- 1640 moderate (Sigma, Germany) supplementedwith 10% FBS (fetal bovine serum) (Sigma, Germany), 100 U/ml penicillin and 100 g/ml streptomycin(Sigma, Germany). and DNA fragmentation checks corroborated the apoptosis inducing activity of GFJ. Consistently, after treating KB cells with GFJ(1g/mL), caspase-3 activity and Bax: Bcl-2 ratio were raised by 7. 35. 6 and (P <0. 001) folds, respectively. Conclusion. The results of the study advanced that GFJ induces apoptosis in the KB cells through increasing caspase-3 activity and Bax: Bcl2 ratio which could be attributed to its organo-sulfurcomponents. Keywords: Garlic, apoptosis, squamous cell carcinoma == Advantages == Today, a considerably growing deal of attention has been dedicated to the use of organic compounds and dietary real estate agents in order to prevent and treatment of a wide variety of medical complications such as cardiovascular disorders and malignancy. 1, 2Life style, in the event of cancer avoidance, is a important factor. To place this into perspective, like a general idea, those who have experienced regular diet programs including enough amounts of fiber and antioxidants less likely will suffer from a particular type of malignancy. 3 Among dietary real estate agents the generally obtainable ingredients that are widely being used by the most of the sides population with significant antioxidant an malignancy preventive potentials are of utmost importance. For example , Allium sativum (Common name: garlic) from friends and family Liliaceae is one of the most valuable edible plants. four, 5The utilization of garlic since food, essence and traditional herbal medicinal backs to more than 4000 years ago. 6 There are a plethora of files that have shed light on the cancer-preventing effect as well as other properties of garlic. 7The pivotal elements responsible for most of the beneficial effects of garlic including its anticarcinogenic activity are known as organosulfur compounds. eight According to meta-analyses of epidemiological studies garlic intake conversely correlates with the incident of a few cancers. This conclusion was 360A drawn that in individuals who consumed garlic regularly, gastric, stomach and colon cancers are considerably reduced compared to those who by no means or infrequently consumed it. 9 In a double-blind randomized clinical trial (RCT) in patients with colorectal adenomas a significant restorative effect was observed after 12 months usage of elderly garlic draw out. In this research the size and the number of tumors in these individuals noticeably reduced. 10 Furthermore, after treating with the essential oil ofA. sativum(0. 360A 11 g/mL) complete inhibition was discovered on the development and 360A mycotoxin biosynthesis in the fungusAspergillusversicolor. With this study, the effects of garlic petrol were attributed to its main KIFC1 compounds that have been consisted of organosulfurs: diallyl-disulfide, diallyl-trisulfidemethylallyl-trisulfide, and methyl-allyl-disulfide. 11 A new organosulfur substance, garlicnin A, which was isolated from acetone extract of garlic was shown to control proliferation of tumor cellsthrough suppression 360A in the tumor connected macrophages. 12 As there is absolutely no report within the effects of garlic on the squamous cell carcinoma, the current in vitro research was designed based on the evidence pertaining to the indisputable anticancer potential ofA. sativumthat prompted us to investigate the apoptotic effect of its new juice within the human dental squamous cell carcinoma cell line (KB). == Components and Methods == == Plant Components == New garlic was purchased coming from retail green grocery stores in Tabriz, Iran, and then was identified in the herbarium of pharmacognosy, Pharmacology Department, Tabriz University of Medical Sciences. On the day of experiments the bulbs of garlic were peeled and ground to 360A get a fresh juice. Then the new juice was homogenized and was passed through 2 mm filters to become used for the experiments. == Total Sulfur Content of Garlic Juice == To confirm the presence of sulfur containing substances and to quantify the total sulfur content in the GFJ, a method previously referred to by Keisset al13was performed. The total sulfur content was measured in 1 mL of GFJ spectrophotometrically according to the abovementioned method. Calibration was done usingpotassium sulfate (K2SO4). == Cell Culture == KB cells (oral squamous cell carcinoma) were purchased from National Cell Traditional bank (Pasteur Company, Tehran, Iran). After Cell growthinRPMI- 1640 medium (Sigma, Germany) supplementedwith 10% FBS (fetal bovine serum) (Sigma, Germany), 75 U/ml penicillin and 75 g/ml streptomycin(Sigma, Germany). Incubation ofcells were.